We use a variety of specialized techniques. We have published many of these methods in peer reviewed journals so other labs can benefit from our optimizations.
Electrophysiology
We are a core member of the Membrane Transporter Expression Facility (MTEF). This is a one of a kind facility in Australia. This is frequently used in our research, for collaboration and also run as a service. Contact Matt for more details.
Contains:
A Xenopus oocyte colony
Several Two-electrode voltage clamp (TEVC) set-ups
Patch clamp electrophysiology
Microelectrode Ion Flux Estimation (MIFE)
Single cell and whole tissue pressure probe
Membrane potential and ion concentration measurement
Simultaneous flux and current measurement
Plant growth
Hydroponics growth of Arabidopsis
Hydroponic growth of fruiting grapevine in controlled conditions to analyse reproductive performance and physiology
Tissue culture facilities
Access to extensive growth room and greenhouse facilities including the Plant Accelerator
Single cell analysis
In situ PCR (protocol in review)
Single cell sampling and analysis (SiCSA) for ion concentrations and expression analysis - example 1, example 2, example 3.
Transient transformation of Arabidopsis mesophyll protoplasts for protein localisation and protein-protein interactions
Aequorin for plant calcium concentration measurement
Confocal microscopy
Standard plant molecular biology techniques (qPCR, plant transformation, cloning, heterologous expression for functional analysis)
